Research on Curcumin Inhibition HepG2 Human Hepatoma Cells

Abstract

In recent years, with the deepening of the understanding of cancer, it has been recognized that cancer can be prevented
through specific diets. Numerous in vitro and in vivo experimental models have also revealed that Curcumin regulates
cellular signaling pathways effects of Curcumin likely activate cell death signals and induce apoptosis in cancer cells,
thereby inhibiting the progression of the disease. In this study, the impact of Curcumin on the expression level of P53
protein in HepG2 human hepatoma cells was observed to explore its inhibitory effect and apoptotic induction of HepG2I
and its mechanism. In this study, the MTT method will be used to detect the effect of Curcumin on the proliferation
of HepG2 human liver cancer cells, and the regression calculation of Curcumin IC50 will be conducted according to
the concentration and inhibition rate. The western blot method will be used to detect the effect of ginger and flavin on
the expression of wild-type p53 in tumor cells. The effect of Curcumin on Bax apoptosis protein expression in HepG2
human hepatoma cells was detected by pFT-A, a p53 inhibitor.