This paper explores homotopy groups, a central topic in algebraic topology, and provides a detailed examination of their foundational role in understanding the structure of topological spaces. Beginning with the definitions and operations of homotopy groups, the discussion progresses to the construction of a chain complex and a rigorous proof of its exactness. The paper delves into the application of homotopy theory to fibrations, deriving an exact sequence that elucidates the intricate relationship between loop spaces and the fundamental group in simply connected spaces. This relationship underscores how fundamental groups can be interpreted through the lens of homotopy, particularly in the context of loop spaces. The theoretical results are further applied to algebraic varieties and schemes, highlighting the broader implications of homotopy theory in areas such as algebraic geometry. By investigating how homotopy groups influence the topological structure of algebraic varieties and their associated schemes, the paper demonstrates the significant utility of homotopy theory in connecting abstract topological concepts with concrete algebraic structures. The challenges of working with exact sequences, particularly in the complex landscape of higher homotopy groups, are also addressed, underscoring the mathematical sophistication required to navigate these topics. This exploration provides valuable insights into the role of homotopy theory in modern mathematical analysis, emphasizing its deep and far-reaching impact across various fields.